solve the structure. Trial and error, as usual.
Mark
-Original Message-
From: Acoot Brett
To: CCP4BB
Sent: Fri, Jan 3, 2014 9:23 pm
Subject: Re: [ccp4bb] DNA Protein co- Crystallization
Dear All,
For the question, I think for a protein-DNA interaction, the protein may
interact w
We have limited experience in protein:DNA crystallization (N=1), but this is
what worked for us a few years ago.
Like others have advised, I also suggest using oligos with different ends. We
tested two protein constructs, one with a a TEVP cleavable N-terminal His tag
and the other with a C-t
Dear All,
Thank you very much for your valuable suggestions.
Venkat
On Sat, Jan 4, 2014 at 9:53 AM, Acoot Brett wrote:
> Dear All,
>
> For the question, I think for a protein-DNA interaction, the protein may
> interact with any sequences of DNA, which will give a lot of combination of
> prote
Dear All,
For the question, I think for a protein-DNA interaction, the protein may
interact with any sequences of DNA, which will give a lot of combination of
protein-DNA sequence for crystallization screening. Or do anyone regard to
just try the crystallization of the protein-one specific seq
My suggestions are
1 try complementary and non-complementary overhangs which can form
Watson-crick and/ or Hoogstein base pairing.
2. If it binds self-complementary duplexes then try them also.
3. Peg conditions with slight acidic pH are more suitable.
4. Divalent cation salts (Ca, Mg, Mn) in cryst
co-factor. If you replace Mg
with Ca, your complex may be more stable/robust and the crystallization may
work better.
Hope this helps.
Mark
-Original Message-
From: venkatareddy dadireddy
To: CCP4BB
Sent: Fri, Jan 3, 2014 4:26 am
Subject: [ccp4bb] DNA Protein co
venkatareddy dadireddy
Sent: 03 January 2014 11:16
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] DNA Protein co- Crystallization
Hi,
I'm working on DNA binding protein, looking to co-crystallize protein- DNA
complex and have no previous experience. Your suggestion would be very precious
on the foll
Hi,
I'm working on DNA binding protein, looking to co-crystallize protein- DNA
complex and have no previous experience. Your suggestion would be very
precious on the following queries.
1. My protein is 646 amino acid long and it exists as homodimer. It is also
having around 20 amino acid e
