dified cysteine
> intramolecularily.
>
> Dhiraj
> --
> *From:* Jon Cooper
> *Sent:* Sunday, November 1, 2020 7:41 AM
> *To:* Srivastava, Dhiraj ;
> CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] Cysteine oxidation product
>
> Hello,
>
>
Subject: Re: [ccp4bb] Cysteine oxidation product
Hello,
does the protein you added cysteine to (I assume you mean by mutagenesis) have
another cysteine which could make an intra molecular disulphide with it? That
would probably not show on non-reducing SDS?
Best wishes, Jon Cooper
Hello,
does the protein you added cysteine to (I assume you mean by mutagenesis) have
another cysteine which could make an intra molecular disulphide with it? That
would probably not show on non-reducing SDS?
Best wishes, Jon Cooper
Original Message
On 31 Oct 2020, 15:58, Sri
iraj
Sent: Saturday, October 31, 2020 4:58:37 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Cysteine oxidation product
Hi All
I added an extra cysteine residue to one of my protein interactions
partners and somehow it increased the affinity in the absence of reducing agent
however in the p
94793-284
From: CCP4 bulletin board on behalf of Srivastava,
Dhiraj
Sent: Saturday, October 31, 2020 4:58:37 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Cysteine oxidation product
Hi All
I added an extra cysteine residue to one of my protein interactions
pa
Hi All
I added an extra cysteine residue to one of my protein interactions
partners and somehow it increased the affinity in the absence of reducing agent
however in the presence of reducing agent, the affinity is same. there is no
disulfide bond formation between the two interaction par
