: Re: [ccp4bb] Cysteine-S-Iodine interaction
On 17/03/2022 17:37, Mohd Syed Ahangar wrote:
>
>
> I have been doing some protein crystal soaking with some covalently
> binding fragments and in one structure I have got an extra density on
> Cysteine but that density doesn't mat
Check the electron density (O, Cl, I ?) and the anomalous signal on
your "iodine".
Cysteine has an interesting chemistry and one is oxidation to the
sulfenic acid, CSO, already mentioned. Probably, it is more common
than we tend to expect and may be overlooked, because we don't look
for it and be
Dear Modh,
I agree with Paul, this is covalente modification of Cys. I would try
cysteine persulfide (CSS), and cysteine sulfenic acid (CSO).
In COOT, center on this cysteine, and then do: Calculate -> Modelling ->
Replace residue; write CSS (or CSO), save the coordinates and refine.
HTH,
Jo
Hi,
it may be oxidized cysteine. Look here:
https://www.researchgate.net/publication/44656220_Protein_S-thiolation_by_glutathionylspermidine_Gsp_The_role_of_Escherichia_coli_Gsp_synthetASEamidase_in_redox_regulation
and here:
Trundle D, Cunningham LW. Iodine oxidation of the sulfhydryl groups of
c
On 17/03/2022 17:37, Mohd Syed Ahangar wrote:
I have been doing some protein crystal soaking with some covalently
binding fragments and in one structure I have got an extra density on
Cysteine but that density doesn't match with the expected fragment.
The fragment was in the form of iodide s
