Re: [ccp4bb] Crystallization problems using MPD (2-Methyl-2,4-pentanediol)

Hi js It happened to us as well with a 1:1 protein complex with decent affinity (Kd about 1 uM). The MPD-based condition (quite high % MPD if I recall correctly) would break the complex apart and only one of the two components formed a crystal (effectively allowing crystallisation of a sample w

Re: [ccp4bb] Crystallization problems using MPD (2-Methyl-2,4-pentanediol)

Hi, If the interaction between partners is of a hydrophobic nature and involves leucines, it may be MPD destroys the interaction. Have a look at the following publication: Acta Crystallogr D Biol Crystallogr. 2002 Oct;58(Pt 10 Pt 1):1722-8. Epu

[ccp4bb] Crystallization problems using MPD (2-Methyl-2,4-pentanediol)

Hi everyone, this is Joerg Stetefeld. I would like to approach the community in a peculiar case of 2-Methyl-2,4-pentanediol (MPD) impacting our crystallisation experiments. We are working on extracellular ligand-receptor complexes, which we characterize in advance to crystallisation att