Hi Sabine,
I vaguely recall that an old colleague had used a mini detergent screen (as
additive) to solve a problem such as yours. It may be worth setting up a plate
or two to screen a few conditions before you embark into more molbio. You can
use the 96 well plates with multiple wells (Corning sel
Hello Sabine,
Maybe this is an option:
Structure, Vol 14, 1617-1622, November 2006
Lysine Methylation as a Routine Rescue Strategy for Protein Crystallization
best regards & good luck
Christian
Schneider Sabine wrote:
>
> Hi everyone,
>
>
>
> I am trying to crystallise an extremely soluble
: Schneider Sabine <[EMAIL PROTECTED]>
Reply-To: Schneider Sabine <[EMAIL PROTECTED]>
Date: Thu, 22 Feb 2007 14:08:44 -
To:
Conversation: Crystallisation of a extremly soluble protein
Subject: [ccp4bb] Crystallisation of a extremly soluble protein
Hi everyone,
I am trying to crystallise
Hello, Sabine.
I believe the technique that Kendall is referring to is Surface Entropy
Reduction, which involves replacing large, highly entropic residues on the
surface of the protein with lower entropy residues to reduce the entropy
shield and facilitate crystallization. Traditionally, the
Sabine,
There are protocols to modify surface residues that can help with
crystallization, and make the protein less soluble. Unfortunately, I¹m
drawing a blank on the details. I remember someone in Andrzej Joachimiak¹s
group was working on this as a rescue approach for the structural genomics
pip
Hi everyone,
I am trying to crystallise an extremely soluble and charged protein. It
is ~30kDa and has an estimated PI of 5.2 and theoretical charge over pH
range 4-10 from + 24 to -29. It is still happy at a concentration of
190mg/ml and fully reconstituted with its ligand.
I have tried h
