Glutaraldehyde works best at low pH
On Mon, Nov 7, 2011 at 8:40 AM, Ed Pozharski wrote:
> On Mon, 2011-11-07 at 05:19 +, Sam Arnosti wrote:
>> Hi everyone
>>
>> I have a protein that is extraordinarily stable at PH=3.0 or even 2.0.
>>
>> I want to crystallize it in the low PH and compare the
I had a protein that loved pH 3 if it was holo (citrate, ammonium bromide,
and PEG) or pH 4 when apo. Those were microbatch conditions rather than
vapor diffusion. If there is no indication of gross structural changes you
might consider different pH soak on your crystals rather than finding a new
c
On Mon, 2011-11-07 at 05:19 +, Sam Arnosti wrote:
> Hi everyone
>
> I have a protein that is extraordinarily stable at PH=3.0 or even 2.0.
>
> I want to crystallize it in the low PH and compare the differences between
> the crystals in regular PH and low PH.
>
> I was wondering how people
I remembered that people had crystallize a series of
streptavidin-2-iminobiotin structures at a low pH. If it might help, check
the following PDBIDs:
2RTD
2RTE
2RTI
2RTK
2RTL
> Hi everyone
>
> I have a protein that is extraordinarily stable at PH=3.0 or even 2.0.
>
> I want to crystallize it in
I have crystallized in PEG with citrate at pH 3. If you want to go lower
I would suggest maleate:
effective pH range pKa 25°Cbuffer
1.2-2.6 1.97 maleate (pK1)
2.2-6.53.13 citrate (pK1)
Enrico.
On Mon, 07 Nov
I'm not convinced that you need a conventional buffer at pH 2 or 3. At pH 2,
the hydrogen ion concentration is 10 mM. If you want to use something else,
the second pKa for sulfuric acid is around 2. The first pKa for phosphoric
acid is slightly higher than 2. Lactic acid has a pKa close to 3
Tendamistat (1OK0) was crystallized at pH 1.3 and diffracted to 0.93A.
George
On Mon, Nov 07, 2011 at 05:19:29AM +, Sam Arnosti wrote:
> Hi everyone
>
> I have a protein that is extraordinarily stable at PH=3.0 or even 2.0.
>
> I want to crystallize it in the low PH and compare the differen
behalf of Sam Arnosti
[meisam.nosr...@gmail.com]
Sent: Monday, November 07, 2011 7:19 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystalization in low PH
Hi everyone
I have a protein that is extraordinarily stable at PH=3.0 or even 2.0.
I want to crystallize it in the low PH and c
Hi everyone
I have a protein that is extraordinarily stable at PH=3.0 or even 2.0.
I want to crystallize it in the low PH and compare the differences between the
crystals in regular PH and low PH.
I was wondering how people set up the boxes in low PH, as usual buffers are
mostly less acidic.
