essage-
From: Prem kumar mailto:pk_ai...@yahoo.com>>
To: CCP4BB mailto:CCP4BB@JISCMAIL.AC.UK>>
Sent: Fri, Apr 13, 2012 5:10 am
Subject: [ccp4bb] Crystal behave funny
Hi all,
I got some Protein + DNA complex crystals (image attached) recently.
They are needle shape some times splitted chromo
: Fri, Apr 13, 2012 5:10 am
Subject: [ccp4bb] Crystal behave funny
Hi all,
I got some Protein + DNA complex crystals (image attached) recently.
They are needle shape some times splitted chromosome type crystals. When we
pick long needles they bend so much than normal crystal but they dont break
aid than done).
>
> - Try to find conditions where the crystals don't start to redissolve while
> you mount them
>
> Original message
>> Date: Fri, 13 Apr 2012 18:51:58 +0800
>> From: CCP4 bulletin board (on behalf of Prem kumar
>> )
>> Subject:
letin board (on behalf of Prem kumar
>)
>Subject: [ccp4bb] Crystal behave funny
>To: CCP4BB@JISCMAIL.AC.UK
>
> Hi all,
> I got some Protein + DNA complex crystals (image
> attached) recently.
> They are needle shape some times splitted chromosome
> type crystals.
Hi Prem,
The first thing I would do is to make certain that you really have prot+DNA
crystals, and not DNA alone. If you can isolate enough crystals (you may need
15 or 30, depending on how large they are), SDS page would be informative. Run
protein and DNA alone and together in the same gel as
