cus Winter (Oxford Diffraction)
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Andy Torelli
Sent: 24 April 2009 16:46
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Cryo-protectant
Hi Liew,
There have already been some very good sugge
Your mother liquor is already very close to being a cryoprotectant. I
think adding 5% ethylene glycol, glycerol, etc. is enough. Too much
may damage your crystals. In addition, you can also try:
1. Grow new crystals in your current crystallization condition + 5%
cryoprotectant.
2. Increase the PE
Title: Re: [ccp4bb] Cryo-protectant
First of all, you need to find out if crystals you grew have similar
quality before you conclude poor diffraction is due to cryoprotectant
solutions. As others have suggested, you can test crystals using
capillary mounting method. Second, there are a lot
Hi Liew,
There have already been some very good suggestions. I agree with Tim
Gruene that a great starting point is to test the diffraction properties
of your crystal at room temperature. This can serve as a baseline for
comparing/evaluating cryoprotecting agents and methods.
You can als
Hi all
Thanks for your precious suggestions and ideas.
The crystallization buffer condition is 0.1M BIS-TRIS pH 5.5, 0.2M MgCl.6H2O,
35% PEG3350
Now, my crystal seem ok in 20% ethylene glycol, but only after a couple minutes
of dehydration at room temperature. For sure, i will try other cryopr
What is the crystallization condition?
On Fri, 2009-04-24 at 11:46 +0800, Liew Chong Wai wrote:
> Hi all,
>
> Good day
> I used MPD as a cryoprotectant (20%, 30%) for my crystal. However,
> there is no diffraction signal at all. Without the MPD cryo, i still
> manage to get 5angstrom, but it ha
I usually try the following, in order:
1. Transfer crystals to mother liquor + 30% glycerol or ethylene glycol
(sometimes lower depending on crystallization solution). This did not
work for you.
2. Transfer crystals to mother liquor + 30% glucose (or try sequential
soaks in M.L. + 15% and then
Hallo,
there is quite a large number of cryo-protectants other than MPD or
glycerol. If I remember correctly, the Methods of Enzymology, (176) list
some of the, and you might check the recipes of the Hampton cryo screen.
As suggested by Li, you can try oils. You can use small sugars (fructose
200031, China
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发件人: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] 代表 Liew Chong
Wai
发送时间: 2009年4月24日 11:47
收件人: CCP4BB@JISCMAIL.AC.UK
主题: [ccp4bb] Cryo-protectant
Hi all,
Good day
I used MPD as a cryoprotectant
Hi all,
Good day
I used MPD as a cryoprotectant (20%, 30%) for my crystal. However, there is no
diffraction signal at all. Without the MPD cryo, i still manage to get
5angstrom, but it has very strong ice-ring signal. I used glycerol (15%, 20%,
25% and 30%) before, but it cracked the crystal.
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