; toufic el arnaout
> School of Medicine - 660 S Euclid Ave
> Washington University in St. Louis
> St Louis, MO 63110, USA
>
>
> From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of R. M. Garavito
> [rmgarav...@gmail.com]
> Sent: Sunday, July 14, 2013 11:17 AM
>
. Garavito [rmgarav...@gmail.com]
Sent: Sunday, July 14, 2013 11:17 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Concentrating purified membrane protein
Raji,
One point the most people forget about is that whenever you concentrate any detergent-solubilized membrane protein is that you w
Raji,
One point the most people forget about is that whenever you concentrate any
detergent-solubilized membrane protein is that you will ALWAYS concentrate the
detergent. So regardless of the MWCO, if the protein-detergent complex
concentrates, the overall detergent concentration also increas
can you elute off the column with low pH ? Or EDTA if you don't want imidazole
around ?
Jürgen
On Jul 14, 2013, at 10:29 AM, Raji Edayathumangalam wrote:
Thanks everyone for your responses. I definitely plan to save the flowthrough
so we'll see what happens. My protein has a His tag and I did
Thanks everyone for your responses. I definitely plan to save the
flowthrough so we'll see what happens. My protein has a His tag and I did
consider doing an affinity step for concentration except I do not want to
have imidazole for some functional assays that I need to carry out with the
protein.
CCP4BB@JISCMAIL.AC.UK] on behalf of Raji
Edayathumangalam [r...@brandeis.edu]
Sent: Sunday, July 14, 2013 1:47 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Concentrating purified membrane protein
Hi Folks,
Sorry for the non-ccp4 post.
I have purified an 18kDa membrane protein and want to conc
ys Grinter
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Raji
Edayathumangalam [r...@brandeis.edu]
Sent: 14 July 2013 01:47
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Concentrating purified membrane protein
Hi Folks,
Sorry for the non-ccp4 post.
I have purified an 18kD
Hi Folks,
Sorry for the non-ccp4 post.
I have purified an 18kDa membrane protein and want to concentrate the
protein from gel filtration fractions, which are in buffer containing 0.05%
DDM (well above the CMC for DDM). My colleague was able to concentrate a
30kDa membrane protein using a 100kDa M
