Re: [ccp4bb] Completely Off-Topic

2017-01-15 Thread Kevin Jude
> > > Reza Khayat, PhD > Assistant Professor > City College of New York > Department of Chemistry > New York, NY 10031 > > > From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK ] > on behalf of Tim Gruene [tim.gru...@psi.ch ] >

[ccp4bb] Completely Off-Topic: Intracellular Glutamate Concentration

2017-01-12 Thread Keller, Jacob
This is one source paper I found recently, and there are lots of others. doi:10.1038/nchembio.186 JPK From: Napoleao Fonseca Valadares [mailto:n...@ifsc.usp.br] Sent: Thursday, January 12, 2017 12:18 PM To: Keller, Jacob Cc: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Completely Off-Topic

Re: [ccp4bb] Completely Off-Topic

2017-01-12 Thread Napoleao Fonseca Valadares
- Mensagem original - De: "Jacob Keller" Para: CCP4BB@JISCMAIL.AC.UK Enviadas: Quarta-feira, 11 de Janeiro de 2017 22:45:03 Assunto: [ccp4bb] Completely Off-Topic Dear Crystallographers, Was anyone else aware that in E coli the intracellular glutamate concentration is ~100

Re: [ccp4bb] Completely Off-Topic

2017-01-12 Thread Harry Powell
Can I just make the point that the subject "Completely off-topic" is completely useless if you're searching through the archives for anything? Since it's attracted quite a bit of correspondence, this obviously isn't off-topic at all! Harry -- Dr Harry Powell Chairman of International Union of

Re: [ccp4bb] Completely Off-Topic

2017-01-12 Thread David Schuller
I wonder if anyone has tried glutamate as a cryprotectant for proteins. If the [] is that high, it may make a stable environment for proteins. I have no idea what effect it might have on ice formation. https://amb-express.springeropen.com/articles/10.1186/2191-0855-3-36 /Bacillus subtilis/ n

Re: [ccp4bb] Completely Off-Topic

2017-01-12 Thread Jon R Sayers
> [tim.gru...@psi.ch] > Sent: Thursday, January 12, 2017 3:55 AM > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] Completely Off-Topic > > Dear JPK, > > I was not aware of the absolute numbers, but maybe they are little suprising: > when your tinned food contains 'yeast extr

Re: [ccp4bb] Completely Off-Topic

2017-01-12 Thread Reza Khayat
rd [CCP4BB@JISCMAIL.AC.UK] on behalf of Tim Gruene [tim.gru...@psi.ch] Sent: Thursday, January 12, 2017 3:55 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Completely Off-Topic Dear JPK, I was not aware of the absolute numbers, but maybe they are little suprising: when your tinned food conta

Re: [ccp4bb] Completely Off-Topic

2017-01-12 Thread Tim Gruene
Dear JPK, I was not aware of the absolute numbers, but maybe they are little suprising: when your tinned food contains 'yeast extract' it is equivalent to monosodium glutamate, which is commonly used as flavour enhancing agent. I am not a chemist to worry about it, but yeast seems to have a ful

[ccp4bb] Completely Off-Topic

2017-01-11 Thread Keller, Jacob
Dear Crystallographers, Was anyone else aware that in E coli the intracellular glutamate concentration is ~100 mM? Also other cell types (yeast, mammalian) are 10s mM. Anything to say about this? I learned of this just recently, and have been amazed about it for more than a week. Did I miss thi