Dear Antonio,
we have seen this type of modifications in some of our structures. The
modification of the cysteine (to cysteine-sulfenic, sulfinic or sulfonic acid)
usually arises from exposure to oxygen during crystallization. We managed to
prevent this by either adding TCEP to the protein buffe
t: Wednesday, May 17, 2017 4:04 PM
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] CYS modification and choice of PEG
I haven't asked anything for a loong time, so here are a couple of question
"for the honourable members of the esteemed CCP4 bulleti
AIL.AC.UK
Subject: [ccp4bb] CYS modification and choice of PEG
I haven't asked anything for a loong time, so here are a couple of question
"for the honourable members of the esteemed CCP4 bulletin board" ... or as I'd
usually say: "for y'all". ;)
1) I
On Wednesday, 17 May, 2017 20:03:42 Antonio Ariza wrote:
> I haven't asked anything for a loong time, so here are a couple of
> question "for the honourable members of the esteemed CCP4 bulletin board" ...
> or as I'd usually say: "for y'all". ;)
>
> 1) I have this modified CYS in one of t
