Dear All;
Thank you very much for your comments and advices.
My protein is homo-tetramer. Based on difference density, only one cysteine
(among the four catalytic cysteins) has observed two positive density along
SG-group. This one could be modeled into OCS, while the rest will be
modeled as non-
Dear Uma
Your modeling of a Cysteine sulfenic group (SOH) is reasonable given the
observed difference density but do keep in mind that sulfenic groups are very
susceptible to further oxidation to sulfinic and sulfonic acids.
Stabilization of sulfenic and sulfinic groups in enzyme active sites is
Dear Uma,
You can do this using coot. Go to Extensions > Modelling > Replace
Residue... and enter the three letter code.
Cheers
Hugo Correia
2012/5/4 Uma Ratu
> Dear All:
>
> My protein has a key cysteine residue involved in catalytic activity.
>
> The template structure used for the modeling
Uma,
It is possible you have either S-hydroxycysteine (CSO) or S-oxycysteine
(CSX). The only difference, crystallographically, is that the CSO S-O
bond is about 1.78A and the CSX S-O bond is about 1.50A. You may not be
able to differentiate this difference in your electron density maps. A
SH
alf Of Uma Ratu
Sent: Friday, May 04, 2012 10:00 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] CSX
Dear All:
My protein has a key cysteine residue involved in catalytic activity.
The template structure used for the modeling has the same key cysteine. In the
template structure, this key cysteine
