Dear Geoffrey,
it seems you read the manual
(http://www.ysbl.york.ac.uk/~emsley/coot/doc/chapters/user-manual_5.html#SEC137),
but you didnt replace the imol and master-chain-id with a value. You
want to specify the molecule (imol, e.g. 0) and the chain from which to
copy to others (e.g. "A"),
This post has a question and an answer (good karma)...answer first
In response to Matt's question about periplasmic harvesting, we do an
osmotic lysis of e.coli by a series of centrifugations. First, harvest your
cells normally (4k 15 min). Then vigorously resuspend in a 20% (w/v)
sucrose, 1 mM ED
