I incubated my pelets in 8M urea for a couple of hrs at 37C, spun and
collected the supernatant, and then refolded the protein over a Ni column,
as I had a his tag protein. To do this, you load and wash the protein
normally, but before eluting, run b-cyclodextrin (i think 0.1%, you'll
have to t
Refold protein 8M Urea:
Sanjiv,
The recommendations for rapid dilution and trying 6M GdmCl are great
recommendations. As an alternative, I had a similar problem years ago with a
membrane-associated receptor domain that was mostly found in inclusion
bodies after expression (I assume this is your
