this e-mail
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of F.
Timur Senguen
Sent: 18 July 2011 14:53
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Bypassing phase separation for nice crystals.
Hi everyone,
I have been issues with a particular protein. I have been clo
Thanks for all the suggestions.
I forgot to mention that I had tried microseeding and it was partially
successful. I am trying this while also pursuing other venues. At Florian's
suggestion I have set up a free interface diffusion method and it looks very
promising. I have a few free floating crys
Original-Nachricht
Betreff:Re: [ccp4bb] Bypassing phase separation for nice crystals.
Datum: Mon, 18 Jul 2011 17:01:27 +0200
Von:Florian Sauer
An: F. Timur Senguen
CC: CCP4BB@JISCMAIL.AC.UK
Dear Timur,
one possibility to handle this problem can be
Hi Timur,
have you tried seeding from your microstalline stuff? Might be worth to try!
Cheers,
Albert
Albert GUSKOV (Dr) | Research Fellow | Division of Structural &
Computational Biology | Nanyang Technological University
Proteos 7-01, Biopolis Drive 61, Singapore 138673 Tel: (65) 6586-9690 GMT+8h
Hi everyone,
I have been issues with a particular protein. I have been close for a while,
but yet so far.
Rather than going from a clear drop to crystal, my protein first undergoes
phase separation (large oily drops) in which one phase contains most, if not
all, of the protein. This phase separat
