Re: [ccp4bb] Blob Game

2015-02-23 Thread Oliver Smart
on 23/2/15 6:57 PM, jens Preben Morth wrote: > a horrible water molecule would fit in there :-) > > On 23/02/15 19:42, Keller, Jacob wrote: >> A little more info: >> >> -Resolution is 1.75 Ang at edge of detector, but I/sig was still ~3; > pushing integration to corners gives 1.66 Ang, but comple

Re: [ccp4bb] Blob Game

2015-02-23 Thread Keller, Jacob
Just a little mother liquor. I am seeing what SO4 does in refinement right now. JPK -Original Message- From: Boaz Shaanan [mailto:bshaa...@bgu.ac.il] Sent: Monday, February 23, 2015 2:50 PM To: Keller, Jacob; CCP4BB@JISCMAIL.AC.UK Subject: RE: [ccp4bb] Blob Game Boaz Shaanan, Ph.D

Re: [ccp4bb] Blob Game

2015-02-23 Thread Boaz Shaanan
@JISCMAIL.AC.UK] on behalf of Keller, Jacob [kell...@janelia.hhmi.org] Sent: Monday, February 23, 2015 8:53 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Blob Game >Ethanol? You weren't setting up drops with liquor on your breath, were you >I can't remember. Ahah, so you did

Re: [ccp4bb] Blob Game

2015-02-23 Thread jens Preben Morth
-Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Keller, Jacob Sent: Monday, February 23, 2015 12:21 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Blob Game Dear Crystallographers, I've got a strange blob hanging off an arginine--see attached. Any

Re: [ccp4bb] Blob Game

2015-02-23 Thread Keller, Jacob
>Ethanol? You weren't setting up drops with liquor on your breath, were you? I can't remember. JPK On 02/23/2015 12:20 PM, Keller, Jacob wrote: > Dear Crystallographers, > > I've got a strange blob hanging off an arginine--see attached. Any ideas? > Nothing weird in the prep; cryst+prot conditi

Re: [ccp4bb] Blob Game

2015-02-23 Thread Edward A. Berry
Ethanol? You weren't setting up drops with liquor on your breath, were you? On 02/23/2015 12:20 PM, Keller, Jacob wrote: Dear Crystallographers, I've got a strange blob hanging off an arginine--see attached. Any ideas? Nothing weird in the prep; cryst+prot conditions: NH4SO4, TRIS/HEPES, CaCl2

Re: [ccp4bb] Blob Game

2015-02-23 Thread Keller, Jacob
, Jacob Sent: Monday, February 23, 2015 12:21 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Blob Game Dear Crystallographers, I've got a strange blob hanging off an arginine--see attached. Any ideas? Nothing weird in the prep; cryst+prot conditions: NH4SO4, TRIS/HEPES, CaCl2, NaCl, L

Re: [ccp4bb] Blob Game

2015-02-23 Thread Keller, Jacob
: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Blob Game Dear Crystallographers, I've got a strange blob hanging off an arginine--see attached. Any ideas? Nothing weird in the prep; cryst+prot conditions: NH4SO4, TRIS/HEPES, CaCl2, NaCl, Li2SO4. There are really no other side chains which could be