Hi all,
joining this discussion late, however, it might worth mentioning that
Amersham/GE has phased out the good old FPLC and is no longer providing
neither service nor spare parts. Best to my knowledge the same will
happen to the Äkta-FPLC system soon leaving the Explorer and Purifier
their
I prefer HPLC too, and am currently using a Waters LC for all my protein
purifications. I have also used a Dionex Bio-LC in the past, which does
have non-corroding PEEK pump heads. It was a great system.
Peter
On 2/14/07 8:18 PM, "Daniel Anderson" <[EMAIL PROTECTED]> wrote:
> It still seems
Hi folks,
I've been an AKTA user for probably close to ten years, and in this time
I've also used BioRad and several other systems. I'd like to make a couple
of comments since everyone seems to be enjoying this lively discussion :)
0. Choose the device for what *you* need. Some applications are e
(FPLC is a trademark. The marketing hype has been so effective that many
people think that FPLC is a method.)
Well, it is, in a way. The FPLC thingie was a pure marketing genius that
bundled the remarkable technology of Monobeads (AFAIK, there is still no
other company that offers comparable s
It still seems to me strange that Bio-Rad would build a machine that can
reach pressures suitable for reverse-phase chromatography, but then not
include software control for gradual pump acceleration. I tried to
communicate to BioRad corporate suits that many HPLC columns are too
fragile for abrupt
In my old lab everyone was slowly converting to Bio-rad for
chromatography. We had an Akta and subsequently bought two Bio-rad
DuoFlow. I actually never made the conversion then but those that had
didn't want to use the Akta anymore.
Upon starting my own lab I got quotes for both an Akta an
That was cryptic.
My point was that the BioRad pumps are very reliable given some basic
care. Its users quickly learn how to use it, as mentioned previously.
On Wed, 14 Feb 2007, Daniel Anderson wrote:
> My direct experience is with the old "HR" pumps, but my remarks apply to
> the newer "DuoFl
My direct experience is with the old "HR" pumps, but my remarks apply to
the newer "DuoFlow".
On Day Zero, I disassemble the pump heads in the order specified in the
manual. I re-assemble with grease on the threads, thus preventing
expensive repairs due to corrosion of the bolts caused by salty bu
I beg to differ.
I have been an AKTA explorer user for about 5 years and switched to
DuoFlow about 4 yrs ago and now have one in my own lab. The BioRad
DuoFlow is awesome. It never ever breaks. I never had any problems
with this machine at all, it takes any column (pharmacia or
otherwise)
Dear all,
Thanks a lot for all the feedbacks on AKTA prime. They are so helpful that I
have abandoned the idea of buying one. Now it is a choice between AKTA FPLC and
Bio-rad DuoFlow. I heard that DuoFlow is not as robust as AKTA and that its
parts break down often. The question is whether qua
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