Dear Chandra
SLS and ourselves (SOLEIL) are dealing rather often with large unit cells and
fancy crystal orientations. No great secret, and things have already been
mentioned: combining three-axis goniometry, large area Pixel detector, low
background, small wedges and/or helical scans, Staranis
We also described how to bend the loops in this article: http://doi.org/gcb8j3
Figure 4 specifically.
On 21/08/2019 18:21, Edwin Pozharski wrote:
In the absence of such you can resort to carefully bending the loop or bending
the pin (Jim Holton made a nifty device for bending the pin) while
>
>
> In the absence of such you can resort to carefully bending the loop or
> bending the pin (Jim Holton made a nifty device for bending the pin) while
> keeping the xtal bathed in the cold stream.
>
>
I would also mention these
https://hamptonresearch.com/product-Adjustable-Mounted-CryoLoop-38
deconvolution procedure has a chance of succeeding.
>
> Colin
>
>
> -Original Message-
> From: CCP4 bulletin board On Behalf Of Edward A. Berry
> Sent: 19 August 2019 17:08
> To: ccp4bb
> Subject: Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] Better Beamline suggest
08
To: ccp4bb
Subject: Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] Better Beamline suggestion!
I would respectfully suggest that higher pixel resolution does not generally
help much in these situations. If an average spot is 10 or more pixels wide
then the profile is defined pretty well. But if the
Hello,
the problem can rather be due to mechanical deformations of the
crystal upon mounting and freezing (bent needle, for example). If
the crystals are needle shaped, aligning the long axis with the
spindle axis and using a beamline with a very small beamsize
"micro
Dear Chandra,
where at APS did you collect? NE-CAT (and in particular beamline 24-ID-E)
is well set-up for long unit cells. You could ask Yury Polikanov (
https://bios.uic.edu/profiles/polikanov-yury/) for advice. He crystallizes
ribosomes with unit cell that are not quite as big as what you ha
I would respectfully suggest that higher pixel resolution does not generally help much
in these situations. If an average spot is 10 or more pixels wide then the profile is
defined pretty well. But if the spots overlap, they still overlap with higher pixel
density. It may make profile fitting
Chandra
What you are looking for here is a beamline with a detector with many pixels
(so you can resolve the long axis) and a multi-axis goniometer - probably a
SmarGon / kappa and an Eiger 16M would make a good combination for this.
Searching on
http://biosync.sbkb.org/
Should allow you to m
Dear All
We recently collected a data set at APS, Chicago with unit cell dimensions of
68.4; 68.4 and 991.6 A. Our diffraction data extends to 3A with the APS set up,
however, the long axis has been problematic, resulting in streaking of the
diffraction data and requires a very specific orienta
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