Hello, a couple of thoughts.
If your best dataset has a twinning fraction of 40% (i.e. almost 50:50 ;-?) and
the twin operator corresponds to a 2-fold rotation parallel to the z-axis, is
it definitely trigonal, rather than hexagonal? The 2-fold NCS operator that you
found for the d3 domains, is
Hmmm -
not much help, but MR can work with twinned data ..
What's the sequence match between your models and your protein? And do you
expect them to form a dimer?
Presumably you found the d3:d3 dimer using MR? I would be a bit worried
that the twinning could mislead a dimer search - are the two d3
Dear all,
I've a project with two historical datasets (i.e. not sure of when/if I can
reproduce to solve problem by getting better data) that are twinned.
The spacegroup is P32, with operator -h, -k, l
cell = 83.5 83.5 64.2 90 90 120
protein has three domains d1-d2-d3, likely 50% solvent, 2
