Hi Ethan,In my understanding, indeed. Pretty much only 2nd domain solves the
structure. When I compare with isolated 2nd domain structure, which is ~half
MW, x-tal grown in different condition plus slightly higher res (2.4A), it's
the same! Only difference is domain1+2 is 3A with poor Wilson B
On Monday, 14 September 2020 22:22:34 PDT Rezaul Karim wrote:
> Hi Eleanor,Thanks for asking.
> Yes, 2nd domain refine alright with Rfree ~25%, inhibitor at the binding site
> and no major issues at all except that I don't see any trace of 1st domain
> and the protein from dissolved crystals are
Hi Eleanor,Thanks for asking.
Yes, 2nd domain refine alright with Rfree ~25%, inhibitor at the binding site
and no major issues at all except that I don't see any trace of 1st domain and
the protein from dissolved crystals are on SDS gel at right MW size = 1st+2nd
domain.I tried all other possib
Hi Randy,
> On 14 Sep 2020, at 18:52, Randy Read wrote:
>
> Thanks for pointing that out. I guess I hadn’t presumed to brand myself as
> an expert! I’ll have to find out now what else I’ve been missing...
Surely you should identify yourself as developer and have access to all the
options!
Hi Huw,
Thanks for pointing that out. I guess I hadn’t presumed to brand myself as an
expert! I’ll have to find out now what else I’ve been missing...
Randy
-
Randy J. Read
Department of Haematology, University of Cambridge
Cambridge Institute for Medical Research Tel: +44 1223 336500
given the domains you already found.
>
> Best,
> Herman
>
>
> Von: CCP4 bulletin board Im Auftrag von Andrew
> Lovering
> Gesendet: Montag, 14. September 2020 11:17
> An: CCP4BB@JISCMAIL.AC.UK
> Betreff: [EXTERNAL] Re: [ccp4bb] Best protocols to advance a low r
t, but Phaser has to option to search
for additional domains, given the domains you already found.
Best,
Herman
Von: CCP4 bulletin board Im Auftrag von Andrew Lovering
Gesendet: Montag, 14. September 2020 11:17
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [EXTERNAL] Re: [ccp4bb] Best protocols to advance a
Rezaul: You say
*But I only see the 2nd domain with exact same unit cell that I have solved
for 2nd domain's with same space group. *
I am afraid if that is so that you probably have only crystallised the 2nd
domain. Does that domain refine all right?
Eleanor
On Mon, 14 Sep 2020 at 09:02, Rezaul
To follow on from this thread:
To answer Jon, I did try to see if P6 subgroups were a possibility but can rule
this out for a few reasons (MR doesn't give solutions, merging stats not
suggestive of P6, the other dataset with the twin fraction that is
significantly further from 50:50); and the d
Eagerly following >I have a similar case. A 3A data with P3(1)21 from two
domains (80+% sequence identity) protein. No major twinning issue. But I only
see the 2nd domain with exact same unit cell that I have solved for 2nd
domain's with same space group. No proteolysis- crystals give protein b
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