[ccp4bb] Baculovirus expression system

2022-02-09 Thread Srivastava, Dhiraj
Thank you every one for all your input. Our concern was whether there is any new advanced system than Bac-to-Bac or Bac-to-Bac is still pretty standard system for baculovirus expression. Based on all your inputs, it appears that we are not very outdated in our information about baculovirus expre

Re: [ccp4bb] Baculovirus expression system

2022-02-07 Thread Artem Evdokimov
There are several but if you discount the transfection based ones (i.e. direct DNA, no virus at all) then the speed is roughly the same - about four weeks from clone to process. FlashBac works a bit faster because it avoids the use of ecoli to recombine the bacmid but the time savings is about a we

Re: [ccp4bb] Baculovirus expression system

2022-02-07 Thread Anshul Assaiya
Please follow the link https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/baculovirus-expression-system On Mon, 7 Feb 2022, 21:54 Srivastava, Dhiraj, wrote: > Hi All > sorry for the question not related to crystallography. What is > the baculovirus express

Re: [ccp4bb] Baculovirus expression system

2022-02-07 Thread Antony Oliver
Date: Monday, 7 February 2022 at 16:24 To: "CCP4BB@JISCMAIL.AC.UK" Subject: [ccp4bb] Baculovirus expression system Hi All sorry for the question not related to crystallography. What is the baculovirus expression system that people use these days to get good yi

[ccp4bb] Baculovirus expression system

2022-02-07 Thread Srivastava, Dhiraj
Hi All sorry for the question not related to crystallography. What is the baculovirus expression system that people use these days to get good yield in less time? Thank you Dhiraj To unsubscribe from the CCP4BB l