Dear H.Sin,
These references may help you:
Meireles, M., Aimar, P., and Sanchez, V. (2004) Albumin denaturation during
ultrafiltration: effects of operating conditions and consequences on membrane
fouling, Biotech. Bioeng. 38, 528-534.
Schratter, P., (2004) Purification and concentration by ult
@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] BSA as additive
I haven't used BSA, but I did recently use 50mM L-glutamic acid for this exact
reason (and 5% glycerol in all buffers except the last one for crystallography)
after reading this paper and it made a big difference to my last protein prep:
tamic acid
sample has fewer, hoping some other drops will yield better crystals over time.
Hope that helps!
Nicola
From: CCP4 bulletin board on behalf of Ha Sin
Sent: 08 May 2017 12:32:44
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] BSA as additive
Dear C
From: CCP4 bulletin board on behalf of Ha Sin
Sent: 08 May 2017 13:32:44
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] BSA as additive
Dear CCP4BB members,
Sorry about my non crystallography question. Does anyone know of a reference
where BSA has been used as an "additive" in the protein c
8 May 2017 13:32:44
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] BSA as additive
Dear CCP4BB members,
Sorry about my non crystallography question. Does anyone know of a reference
where BSA has been used as an "additive" in the protein concentration step to
prevent aggregation of th
Dear CCP4BB members,
Sorry about my non crystallography question. Does anyone know of a
reference where BSA has been used as an "additive" in the protein
concentration step to prevent aggregation of the main protein?
I would appreciate your suggestions.
Thank you,
H.Sin
