Re: [ccp4bb] Fwd: [ccp4bb] B-factor blurring

2014-11-19 Thread Tim Gruene
g to my request below. > > To be explicit, my question relates to B-factor blurring (+B correction), not > to B-factor sharpening (-B correction). > > thanks > > Mike > > > Begin forwarded message: > >> From: Mike Lawrence >> Subject: [ccp4bb

[ccp4bb] Fwd: [ccp4bb] B-factor blurring

2014-11-18 Thread Mike Lawrence
My sincere thanks to all who are responding to my request below. To be explicit, my question relates to B-factor blurring (+B correction), not to B-factor sharpening (-B correction). thanks Mike Begin forwarded message: > From: Mike Lawrence > Subject: [ccp4bb] B-factor blurring

Re: [ccp4bb] B-factor blurring

2014-11-18 Thread Ho Leung Ng
Another useful reference: Liu C & Xiong Y (2014). Electron Density Sharpening as a General Crystallographic Technique. *J. Mol. Biol.* 426, 980-993. http://www.ncbi.nlm.nih.gov/pubmed/24269527 Ho Leung Ng University of Hawaii at Manoa Assistant Professor, Department of Chemistry h...@hawaii.edu

Re: [ccp4bb] B-factor blurring

2014-11-17 Thread Pavel Afonine
Mike, you can have a look here (from page 52 and on): http://phenix-online.org/presentations/fem_06MAY2014.pdf There it is shown that map kurtosis can be used as an optimization criterion for choosing the best sharpening B. Pavel On Mon, Nov 17, 2014 at 5:01 PM, Mike Lawrence wrote: > Dear

Re: [ccp4bb] B-factor blurring

2014-11-17 Thread Tomas Malinauskas
Hi Mike, my favourite summary on B factor sharpening/blurring is here: Acta Crystallogr D Biol Crystallogr. 2006 Aug;62(Pt 8):923-32. Epub 2006 Jul 18. Considerations for the refinement of low-resolution crystal structures. DeLaBarre B, Brunger AT. Hope that helps, Tomas On Mon, Nov 17, 2014 at

[ccp4bb] B-factor blurring

2014-11-17 Thread Mike Lawrence
Dear all can anyone help me with literatures references to B-factor blurring as a technique to reveal low resolution features in an electron density map? I have seen the poster from Andrea Thorn at http://shelx.uni-ac.gwdg.de/~athorn/pdf/thorn_iucr2014_poster.pdf but was wondering if there wer