[ccp4bb] AW: [ccp4bb] Averaging of different proteins in heteromeric complex

2017-01-11 Thread Herman . Schreuder
nsider not handling them a programming oversight. Best, Herman -Ursprüngliche Nachricht- Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Graham Robinson Gesendet: Mittwoch, 11. Januar 2017 17:37 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] Averaging of

Re: [ccp4bb] Averaging of different proteins in heteromeric complex

2017-01-11 Thread Graham Robinson
Dear Crystallographers, Thank you very much Andrew, David, and Jacob (as well as others outside of the BB) for very helpful input on this problem. It does in fact appear that I have a very nice, statistically disordered structure. In published structures containing statistical disorder authors ha

Re: [ccp4bb] Averaging of different proteins in heteromeric complex

2016-12-16 Thread David Schuller
"The proteins are stoichiometric" Huh? What is the stoichiometry? I'm expecting a ratio here, like 1:1 or some such. I see two possibilities: 1) The two proteins are completely interchangeable in the structure. 2) The two proteins are not interchangeable, but are being crystallographically

Re: [ccp4bb] Averaging of different proteins in heteromeric complex

2016-12-16 Thread Keller, Jacob
: Friday, December 16, 2016 11:45 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Averaging of different proteins in heteromeric complex Dear Crystallographers, I have solved the structure of a complex, which is the average of its component proteins. The problem is as follows: The complex is composed

[ccp4bb] Averaging of different proteins in heteromeric complex

2016-12-16 Thread Graham Robinson
Dear Crystallographers, I have solved the structure of a complex, which is the average of its component proteins. The problem is as follows: The complex is composed of two proteins, which share 67%/84% sequence identity/similarity in the resolvable region. The proteins are stoichiometric (SDS-PA