0:04 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Another difficult MR case
Dear all,
Thank you for your kind expert suggestions, and sorry for the late reply, I'm
still trying all the suggested approaches.
Some comments:
- I can't use buccaneer yet, since I'm still working
Crystals with many copies of a single model are indeed notoriously
difficult to solve by MR. There is often a confusion between crystal
symmetry and non-crystallographic symmetry, but in your case that wont
happen - since the SG is P1.
First thing i would check is the self-rotation function.
Is th
Dear Napoleão,
If you have such a large number of copies of the molecule in the
asymmetric unit, you should perhaps try and see whether there is some
orientational regularity in the way they are arranged by calculating a
self-rotation function. Any such indication might enable you to filter
s
Dear all,
Thank you for your kind expert suggestions, and sorry for the late
reply, I'm still trying all the suggested approaches.
Some comments:
- I can't use buccaneer yet, since I'm still working to resolve the
structure.
- Robyn Stanfield's suggestion of using Shelxe to try to verify the
validi
Dear Napoleão,
Yes, I agree with Phil that it looks like a case where there *is* translational
NCS but it's not being used by Phaser, either because it wasn't automatically
detected (native Patterson peak just under the default limit? more than one
off-origin Patterson peak?) or because Phaser
logy Lab
>> The Francis Crick Institute
>> London, UK
>> ==
>> about.me/david_briggs
>>
>> --
>> *From:* CCP4 bulletin board on behalf of Phil
>> Jeffrey
>> *Sent:* Thursday, August 29, 2019 5:24:48 PM
>> *To:*
gs
> Senior Laboratory Research Scientist
> Signalling and Structural Biology Lab
> The Francis Crick Institute
> London, UK
> ==
> about.me/david_briggs
>
> --
> *From:* CCP4 bulletin board on behalf of Phil
> Jeffrey
> *Sent:* Thursday,
alling and Structural Biology Lab
The Francis Crick Institute
London, UK
==
about.me/david_briggs
From: CCP4 bulletin board on behalf of Phil Jeffrey
Sent: Thursday, August 29, 2019 5:24:48 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Another difficult MR case Are you *sure* there's no
tr
ancis Crick Institute
London, UK
==
about.me/david_briggs
From: CCP4 bulletin board on behalf of Phil Jeffrey
Sent: Thursday, August 29, 2019 5:24:48 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Another difficult MR case Are you *sure* there's no
translational NCS ?
For example
d on behalf of Phil Jeffrey
Sent: Thursday, August 29, 2019 5:24:48 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Another difficult MR case
Are you *sure* there's no translational NCS ?
For example your first molecular replacement solution out of Phenix shows
EULER 293.6 27.7
Are you *sure* there's no translational NCS ?
For example your first molecular replacement solution out of Phenix shows
EULER 293.6 27.7 288.7
FRAC -0.02 0.02 0.02
(that's "first molecule at origin in P1")
and
EULER 294.0 27.9 288.8
FRAC -0.37 0.02 0.02
which is essentially the sa
Hi Napo,
Apart from other things possible (wrong space group is often the
culprit, but it seems like not your case), I would consider a
possibility of crystallizing a different protein than expected.
see "Protein purification and crystallization artifacts: The tale
usually not told"
https:/
Deal all,
Sorry for the long post.
I have a data set obtained from a crystal produced after incubating a
protease with a protein which is mostly composed by an antiparallel beta
sheet. I have tried numerous approaches to solve it, and failed.
Molecular replacement using Phaser, and the protease or
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