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-- Ian
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of William Scott
Sent: 07 August 2008 23:27
To: Juergen Bosch
Cc: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Ammonium citrate tribasic buffer
Wikipedia claims Hasselbac
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>
> -- Ian
>
>> -Original Message-
>> From: [EMAIL PROTECTED]
>> [mailto:[EMAIL PROTECTED] On Behalf Of William Scott
>> Sent: 07 August 2008 23:27
>> To: Juergen Bosch
>> Cc: CCP4BB@JISCMAIL.AC.UK
&
lt
-- Ian
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of William Scott
Sent: 07 August 2008 23:27
To: Juergen Bosch
Cc: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Ammonium citrate tribasic buffer
Wikipedia claims Hasselbach is a misspell
gt; Cc: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Ammonium citrate tribasic buffer
>
> Wikipedia claims Hasselbach is a misspelling:
>
> http://en.wikipedia.org/wiki/Henderson-Hasselbalch_equation
>
> Since Wikipedia has now replaced the social consensus as the fin
Wikipedia claims Hasselbach is a misspelling:
http://en.wikipedia.org/wiki/Henderson-Hasselbalch_equation
Since Wikipedia has now replaced the social consensus as the final
arbiter of truth ...
On Aug 7, 2008, at 3:22 PM, Juergen Bosch wrote:
Just on a side note, can someone clarify why Ha
Just on a side note, can someone clarify why Hasselbach is not
Hasselbalch or vice versa ? Or is that the same guy just somewhere
sometime misspelled and for ever in the records ?
See here:
http://www.britannica.com/EBchecked/topic/261188/Henderson-Hasselbach-equation
Jürgen
On 7 Aug 2008,
Good points!
Not only citrate is inappropriate for buffers in the pH 6.5-7.5 range,
but also (as pointed out by your colleagues at UCSC, but probabbly elsewhere,
polyprotic acids are hard to handle, as their pKa change in a significant
fashion depending on their concentration.
In this case, the H
There are 3 pKa's for citric acid:
3.15, 4.77, and 5.19
so it is going to be a lousy buffer at pH 6.7 to 7.25. Also, you need
to know what concentration your buffer is, and whether that
concentration is with respect to the citrate ion or what. It won't be
tribasic with respect to ammoniu
Dear All
Sorry for non crystallographic query.
Can any body mail me how to prepare Ammonium citrate
tribasic (citric acid triammonium salt) buffer pH 6.7
to 7.25 and also what is the pKa value.
Thanking you in advance
Rajakumara
E. Rajakumara
Postdoctoral Fellow
Strcutural Biology Program
