Dear all,
Thanks for all the advices. The solution worked for me is to
calculate Se-MAD phase and Hg-SIR (use SeMet remote wavelength as
"native") phase separately in SHARP; then combine the phase using CNS
(combine.inp). After density modification in DM, ~2/3 of one molecule
(4 molecules
Hi -
I would try SHARP; or to be exact be tempted to think less and use
autoSHARP with the description of all datasets and only give it the Se
sites for the seMet crystal. The same thing can also be done easily in
Solve. In both cases I would start with unmerged data and let these
program
board on behalf of Jacob Keller
Sent: Thu 7/17/2008 11:23 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Advice for phasing
Wouldn't it make sense to do MIRAS with both the Se and Hg derivatives, and add
in some multi-crystal averaging from the native set? You seem like you are
almost
From: Junyu Xiao
To: CCP4BB@JISCMAIL.AC.UK
Sent: Thursday, July 17, 2008 10:13 AM
Subject: [ccp4bb] Advice for phasing
Hi,
I have a very good native data set. However, the selenomethionyl crystal has
a different space group and always suffers from radiation damage. A
three-wavel
Hi,
I have a very good native data set. However, the selenomethionyl
crystal has a different space group and always suffers from radiation
damage. A three-wavelength data set was still collected, and after
phasing in SHARP, some features can be seen but the map is not good
enough for trac
