ED] On Behalf Of Mischa Machius
Sent: Thursday, June 12, 2008 10:15 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Activity of a mutant enzyme compared to wild type - puzzle
I assume you are talking about a sugar-binding enzyme ;) I have some
aspects to consider in addition to what Artem
Hi Subbu,
Here's my two cents.
Your project sounds really interesting.
You mentioned in your email that the active site and associated waters
in the active site of the wild type and mutant enzymes are
"identical". It's worth noting that even very small (on the 1/10th of
an angstrom level)
On a general note, it is not unusual at all for a random mutation (i.e.,
one not in the active or regulatory site of an enzyme, and not
significantly connected with the catalytic or regulatory mechanism) to
affect the rate constant of an enzyme-catalyzed reaction (kcat or
kcat/Km) by a factor o
I assume you are talking about a sugar-binding enzyme ;) I have some
aspects to consider in addition to what Artem raises. Many effects of
a mutation are not recognizable in a static crystal structure or even
in an NMR structure. For example, it is usually difficult to assess
the thermodyna
Artem
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Narayanan Ramasubbu
Sent: Wednesday, June 11, 2008 8:22 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Activity of a mutant enzyme compared to wild type - puzzle
Dear all:
I have a single residue mutant wh
Narayanan Ramasubbu wrote:
Dear all:
I have a single residue mutant whose enzyme activity is about 50% of
the wild type. Interestingly, the mutation
is in a region that involves a secondary site but not the active site.
The two structures with or without ligands
fit well (0.18 A) and the metal
Dear all:
I have a single residue mutant whose enzyme activity is about 50% of the
wild type. Interestingly, the mutation
is in a region that involves a secondary site but not the active site.
The two structures with or without ligands
fit well (0.18 A) and the metal binding and cofactor binding
