Hi Vanessa
It is better to get rid of traces of residual acrylamide that may
contaminate your final purified and refolded RNA.
It is usual to have contaminations with monomeric acrylamide. NMR
spectroscopists studying RNA can usually detect its presence on their
spectra.
If you can dialyze your pur
Are you talking about chunks of acrylamide? Or perhaps trace molecules?
As for chunks, we normally pass it through a 0.2 um filter before
setting up trays.
FR
On Apr 1, 2009, at 4:48 PM, vanessa delfosse wrote:
Dear all,
I'm currently trying to crystallize a 70 nt RNA and I would like to
Dear all,
I'm currently trying to crystallize a 70 nt RNA and I would like to know if
the presence of acrylamide, some traces from the purification step, may
interfere with crystallization of nucleic acids ?
Thanks in advance,
Vanessa.
--
Vanessa Delfosse - PhD
U
