Megha,
If you want to prepare your sample at a pH below the pI, you will want
to use a cation column.
Enzymes at pH's below their pI have an overall positive charge. Cation
exchange is a column which has a negative charge, thus it will bind to
positively charged proteins.
I would suggest looking at
Dear all,
our protein is stable in acidic pH at a pI of 6. we do not have any tag in
it and it is expressed in inclusion body form. which ion exchange
chromatography will suit it cation or anion exchange and do i use a strong
or a weak ion excahnge resin.
thanks and regards.
