ontaminant database. What about
putting it in BioRxiv?
Bert
From: CCP4 bulletin board on behalf of Debanu
Sent: 05 April 2017 16:23
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Accidental crystallization of E. coli protein
I think it is worth reporting/publi
I think it is worth reporting/publishing this crystallization and
structure in addition to depositing it in the PDB and
ContaMiner/ContaBase databases.
Items you can consider in reporting it based on the following but can
take a better decision if you complete the structure to deposition
quality:
Hi Mohamed,
I don't recall such a case of a crystallized proteolytically cleaved
contaminant (see our recent summary of contamination cases
http://scripts.iucr.org/cgi-bin/paper?ei5009 , and references therein).
I would certainly recommend depositing the data at the PDB - it may help
others address
This may seem an interesting case, given that protocol fur purification,
and subsequent crystallization is reproducible as well as this enzyme is a
valid drug target for pathogenic strains of E. coli.
Best
Z
On Apr 5, 2017 02:51, "Vipul Panchal" wrote:
> I don't think it is going to be any scien
I don't think it is going to be any scientific story. Even if you think of
publishing what aspect are you going to discuss? What new are you going to
give to the community.
On Wed, Apr 5, 2017 at 2:46 AM, Mohamed Noor
wrote:
> During the crystallization of a totally unrelated protein from a diff
During the crystallization of a totally unrelated protein from a different
bacterium in E. coli, we managed to somehow crystallize an E. coli protein. It
turned out to be only the catalytic domain of an enzyme. Two previous reports
both used recombinant expression of this enzyme followed by limi
