Re: [ccp4bb] About seeding in microbatch under oil crystallization

2010-05-19 Thread Ravindra Makde
we become" Buddha --- On Wed, 5/19/10, wu donghui wrote: From: wu donghui Subject: [ccp4bb] About seeding in microbatch under oil crystallization To: CCP4BB@JISCMAIL.AC.UK Date: Wednesday, May 19, 2010, 5:28 AM Hi ccp4bbers,   I want to know if anyone has any experience about seeding (str

Re: [ccp4bb] About seeding in microbatch under oil crystallization

2010-05-19 Thread wu donghui
Hungerford, Berkshire, RG177HD, UK > > Directors: Peter Baldock, Patrick Shaw Stewart > > http://www.douglas.co.uk/ > > Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 > > Regd. England 2177994, VAT Reg. GB 480 7371 36 > > > > *From:* CCP

Re: [ccp4bb] About seeding in microbatch under oil crystallization

2010-05-19 Thread Harm Otten
Hello Donghui we used a robot to setup the batch crystallization screens with some microseed stock solution and the relevant protein and reservoir stocks. That worked fine. I only mention a collegues work here, so if there are more detailed questions, I will approach him. Good luck, Harm On Wed,

[ccp4bb] About seeding in microbatch under oil crystallization

2010-05-19 Thread wu donghui
Hi ccp4bbers, I want to know if anyone has any experience about seeding (streak seeding or microseeding) in microbatch under oil crystallization. I wonder if the oil might block or wipe away the seeds if cat whisker is used for streak seeding. Thank you for your input ahead. Best regards, Donghu