Re: [ccp4bb] About protein aggregation~!

2015-05-27 Thread zeyaul islam
Hello Gaoyina S. pombe riboflavin synthase has similar situation . It is homotrimeric in solution state but the protein is monomeric in the crystal structure. Gerhardt S, Schott AK, Kairies N, Cushman M, Illarionov B, Eisenreich W, Bacher A, Huber R, Steinbacher S, Fischer M. Studies on the reacti

Re: [ccp4bb] About protein aggregation~!

2015-05-27 Thread Sebastiaan Werten
Dear dpsk, I asked a very similar question here about a month ago, so I'll post a summary of the replies. In our case, the protein was known to exist in a biologically relevant monomer-dimer equilibrium, with the amount of monomer becoming vanishingly small at high concentrations (e.g. in our

[ccp4bb] AW: [ccp4bb] About protein aggregation~!

2015-05-27 Thread Herman . Schreuder
] About protein aggregation~! Hi, Recentely,we determined a monomer protein structure which looks like an dimer in solution via size-exclusion chromatography and SAXS, have you guys met such a situation before? or some references, Thank you for your reading Best wishes,

[ccp4bb] About protein aggregation~!

2015-05-26 Thread dpsk
Hi, Recentely,we determined a monomer protein structure which looks like an dimer in solution via size-exclusion chromatography and SAXS, have you guys met such a situation before? or some references, Thank you for your reading Best wishes,