Re: [ccp4bb] AW: COOT: adding xylose to a plant glycosylation chain

2018-07-05 Thread Bärbel Blaum
Hi Marilyn, if 'some luck' happens not to be the case you may be able to create a good cif file for the covalent protein-sugar bond with the JLigand tool (CCP4 suite). that tool allowed me to connect an isoaspartate residue at both ends with my protein chain so that neither coot nor refma

[ccp4bb] AW: COOT: adding xylose to a plant glycosylation chain

2018-07-05 Thread Herman . Schreuder
Hi Marilyn, I have been struggling with carbohydrates in the pre Glyco module era, which was *not nice*. However, the following strategy works reasonably well: 1) Get the XYP monomer with the "get monomer" command. 2) Move the XYP manually in its electron density. This can be very ap