[ccp4bb] AW: [ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum

2017-11-13 Thread Herman . Schreuder
:55 An: Schreuder, Herman /DE; CCP4BB@JISCMAIL.AC.UK Betreff: [EXTERNAL] RE: [ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum "Quality of image" has a lot of parameters, including resolution, noise, systematic errors, etc. I am not aware of a global "quality of imag

Re: [ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum

2017-11-12 Thread Dale Tronrud
On 11/12/2017 6:48 AM, Kay Diederichs wrote: > On Fri, 10 Nov 2017 14:04:26 -0800, Dale Tronrud > wrote: > ... >> >> My belief is that the fact that our spot intensities represent the >> amplitude (squared) of a series of Sin waves is the result of the hard >> work of people like Bob who give u

Re: [ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum

2017-11-12 Thread Kay Diederichs
On Fri, 10 Nov 2017 14:04:26 -0800, Dale Tronrud wrote: ... > > My belief is that the fact that our spot intensities represent the >amplitude (squared) of a series of Sin waves is the result of the hard >work of people like Bob who give us monochromatic illumination. >"Monochromatic" simply mean

Re: [ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum

2017-11-10 Thread Dale Tronrud
On 11/10/2017 1:38 PM, Robert Sweet wrote: > This has been a fascinating thread. Thanks. > > I will dip my oar in the water.  Here are a couple of snippets. > >> Jacob: It was good of proto-crystallographers to invent diffraction as >> a way to apply Fourier Series. > > and > >> Ethan: So here'

Re: [ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum

2017-11-10 Thread Robert Sweet
This has been a fascinating thread. Thanks. I will dip my oar in the water. Here are a couple of snippets. Jacob: It was good of proto-crystallographers to invent diffraction as a way to apply Fourier Series. and Ethan: So here's the brain-teaser: Why does Nature use Fourier transforms rat

Re: [ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum

2017-11-10 Thread Keller, Jacob
>>My understanding is that EM people will routinely switch to diffraction mode >>when they want accurate measurements. You lose the phase information but, >>since EM lenses tend to have imperfections, you get better measurements of >>the intensities. Only to my knowledge in the case of crystal

Re: [ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum

2017-11-10 Thread Dale Tronrud
herman.schreu...@sanofi.com] > Sent: Friday, November 10, 2017 10:22 AM > To: Keller, Jacob ; CCP4BB@JISCMAIL.AC.UK > Subject: AW: [ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum > > At the bottom line, it is the quality of the image, not only the amount of > pixels

Re: [ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum

2017-11-10 Thread Dale Tronrud
t; crystallography-style modelling! > > Jacob Keller > > > > -Original Message- > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Tristan > Croll > Sent: Friday, November 10, 2017 8:36 AM > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb]

Re: [ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum

2017-11-10 Thread Keller, Jacob
richt- Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Keller, Jacob Gesendet: Freitag, 10. November 2017 15:48 An: CCP4BB@JISCMAIL.AC.UK Betreff: [EXTERNAL] Re: [ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum It seems, then, to be generally agreed

[ccp4bb] AW: [ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum

2017-11-10 Thread Herman . Schreuder
board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Keller, Jacob Gesendet: Freitag, 10. November 2017 15:48 An: CCP4BB@JISCMAIL.AC.UK Betreff: [EXTERNAL] Re: [ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum It seems, then, to be generally agreed that the conversion between voxels

Re: [ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum

2017-11-10 Thread Keller, Jacob
CP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum Or a nice familiar 2D example: the Ramachandran plot with 7.5 degree binning, as a grid (left) or with bicubic smoothing (right). Different visualisations of the same data, but the right-hand image us

[ccp4bb] AW: Re: [ccp4bb] Basic Crystallography/Imaging Conundrum

2017-11-10 Thread Herman . Schreuder
In line with Dale's suggestions, I would suggest that you reformat your voxel map into the format of an electron density map and look at it with coot. I am sure it will look much better and much more like the electron density we are used to look at. Alternatively, you could display an bona fide