Re: [ccp4bb] AW: [ccp4bb] High R/Rfree

2017-11-14 Thread Eleanor Dodson
Remember DNA can generate some very strong reflections along the stacking axis. But 2outliers" can mean anything - as Herman says look at the images. Aimless shows you a plot of where they are - and gives a list.. - they may be associated with ice rings? or just diffraction problems. On 14 No

[ccp4bb] AW: [ccp4bb] High R/Rfree

2017-11-14 Thread Herman . Schreuder
Dear Radhika, What reason does Xtriage give for declaring the reflections to be outliers? Too weak, too strong, other reasons? As was mentioned before, what is the resolution of your data? In cases like this, it is always good to have a look at the diffraction images to see if there is some pro

[ccp4bb] AW: [ccp4bb] High R/Rfree after MR

2017-10-13 Thread Herman . Schreuder
Dear GIA, In addition to the anisotropy, I would also check your diffraction images and make sure that there are no (even hardly perceptible) ice rings present. Depending on how the data processing software handled this, they may cause high Rfactors in the range you mentioned. Best, Herman Vo