Artem
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Sridhar Prasad
Sent: Sunday, August 31, 2008 12:28 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] AW: [ccp4bb] hi / cloning stuff
I want to draw attention to some of the early unruly response(
I found out that my glycerol stocks were not alright any more and I had no
plasmid backups (do it!!!). So I had to clone, again.
Hint for the future: Dead glycerol stocks, even dead (say, a year old)
clone on a plate, still contain your plasmid. Do a regular miniprep with
it, transform - 99% o
before you click on the send icon on your computer with derogatory
remarks.
Thanks
Sridhar Prasad
-Original Message-
From: CCP4 bulletin board on behalf of Jan Schoepe
Sent: Sat 8/30/2008 8:52 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] AW: [ccp4bb] hi / cloning stuff
Hi, I think your
Hi, I think your question is quite reasonable no matter what others say about
it. There were even people asking about how to make buffers and then a big
discussion about the HH equation arose. And we should not forget that modern
crystallization starts with cloning ;-) Anyway, you should have gi
