Dear Bulletin Board,
as usual with the bulletin board, I quickly got a number of very useful
suggestions, which I summarize below.
Mick Blaise, Alisa Glukhova and Jacinto suggested to use endo F. Mick said that
in his case always some sugar was left, which in my case would keep my protein
in so
Dear Alisa,
Thank you for your suggestions. The carbohydrate should be N-linked and the
protein was expressed in HEK293 cells. Chances of getting another protein
batch in the short-term are slim, so I am looking for ways to trim existing
carbohydrate chains. I will look into PNGase F.
Best,
H
