Hi Hari,
These vectors are indeed not commercial ones. Unfortunately, I do not have any
of them any more. If I were you, I would directly ask the group where they were
originally made - not sure if they are always reading the ccp4 bulletin board
though. Anyways, here are some more things about
Hary,
Delta-aminolevulinic acid is the classical heme (heme b)
precursor for your experiments. As far as I know, the synthesis of
porphyrin goes very quickly in E. coli, since I tried it once with a
bacterial P450 and got a fat overexpression band after ~2hrs in the
lysate. Also the photometri
