: [ccp4bb] Fwd: undefined edensity blob at glutamine
sidechain
dear all:
I. i am not able to fit trp,
since
1. trp doesnt fit well
2. positive density comes in fo-fc map after refinement
3. this is a soaked crystal str with heavy atom soln, the native one has
perfect density for gln, so mutation to trp
It doesn't look like you left a CN on your gold atom. These things
are pretty much covalently bound.
Dale Tronrud
On 12/10/2013 08:13 AM, PriyankMaindola wrote:
> dear all:
> I. i am not able to fit trp,
> since
> 1. trp doesnt fit well
> 2. positive density comes in fo-fc map after refineme
My first guess was also a metal ion. However, a tryptophan as Fred suggested
cannot be ruled out. A simple preliminary test is to scroll up the contouring
level and look when the contours of the blob disappear. If the contours quickly
disappear, you have something disordered or light. If the con
