Re: [ccp4bb] ATP analogues

2024-07-03 Thread Matthew BOWLER
Dear Reza, the stability of these complexes is variable and depends on many factors including your protein, the pH of the buffer etc. I would recommend aluminium fluoride as the most stable (below pH8) as it is present in solution and has a the highest charge to size ratio so binds tightly in

[ccp4bb] ATP analogues

2024-07-03 Thread Reza Khayat
Hi, Sorry for the non crystallography question. We need to make some ATP analogues (ADP BeFx, ADP MgF3, and ADP AlFx). I have attained protocols from manuscripts for making these; however, am concerned that something important in the protocol may be lacking. Also, how stable are these samples?