without ADP crystallizes.
>
> Best regards,
> Herman
>
> --
> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of *Ni
> shi
> *Sent:* Friday, July 27, 2012 5:17 AM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* [ccp4bb] ADP bin
Sent: Friday, July 27, 2012 5:17 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] ADP binds to the protein in solution but not
show up in the structure
Dear CCP4ers,
We have crystallized a kinase without adding any nuceotide, and
Dear CCP4ers,
We have crystallized a kinase without adding any nuceotide, and the structure
does not show any eletron density at the ATP binding site. Yet most of the
bacterial-purified proteins of this family and also the structures contains
ADP . So we boiled the bacterial-purified protei
