If you notice, I did not say 250 kDa DNA. It will have to be a shorter DNA,
that *behaves* on size exclusion like a 250 kDa protein. I once worked out
the ratio, but don't remember what it ended up being, anymore :)
The advantage is that you can get these standards easily enough in a huge
range of
But this wouldn't correspond to a globular form; 250kDa DNA will appear
larger than a 250kDa globular protein.
On Sat, Feb 19, 2011 at 5:25 PM, Artem Evdokimov
wrote:
> A sufficiently long piece of DNA works OK.
>
>
> On Sat, Feb 19, 2011 at 6:47 PM, Alexandra Deaconescu <
> deac...@brandeis.edu
Pennsylvania School of Medicine
kgu...@mail.med.upenn.edu
215-573-7260 / 267-259-0082
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Alexandra
Deaconescu
Sent: Saturday, February 19, 2011 7:47 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] 250 kDa standard
A sufficiently long piece of DNA works OK.
On Sat, Feb 19, 2011 at 6:47 PM, Alexandra Deaconescu
wrote:
> Dear ccp4bb enthusiasts:
>
> A question unrelated to ccp4: can anyone recommend a good 250 kDa standard
> for gel filtration that is commercially available? It could be a single
> polypeptid
Dear ccp4bb enthusiasts:
A question unrelated to ccp4: can anyone recommend a good 250 kDa
standard for gel filtration that is commercially available? It could be
a single polypeptide or an oligomer too...
Thanks a lot!
Bests,
Alex
