Re: [ccp4bb] [Fwd: Re: [ccp4bb] I/sigmaI of >3.0 rule]

2011-03-04 Thread Kay Diederichs
Am 04.03.2011 11:11, schrieb Kay Diederichs: There is nothing wrong with R_meas of 147.1% since, as others have said, R_meas is not limited to 59% (or similar) as a refinement R-factor is. Rather, R_meas is computed from a formula that has a denominator which in the asymptotic limit (noise) appr

Re: [ccp4bb] [Fwd: Re: [ccp4bb] I/sigmaI of >3.0 rule]

2011-03-04 Thread Maia Cherney
Kay, Thank you for your explanation. The radiation damage was not the factor, but there was something strange about this crystal (actually two crystals had the same strange behavior). I could not process them in HKL2000, but it showed the problem (see pictures in the attachment). The processi

Re: [ccp4bb] [Fwd: Re: [ccp4bb] I/sigmaI of >3.0 rule]

2011-03-04 Thread Kay Diederichs
Maia, provided radiation damage is not a major detrimental factor, your data are just fine, and useful also in the high resolution shell (which still has of 2.84 so you could probably process a bit beyond 2.25A). There is nothing wrong with R_meas of 147.1% since, as others have said, R_mea

[ccp4bb] [Fwd: Re: [ccp4bb] I/sigmaI of >3.0 rule]

2011-03-03 Thread Maia Cherney
Original Message Subject:Re: [ccp4bb] I/sigmaI of >3.0 rule Date: Thu, 03 Mar 2011 10:43:23 -0700 From: Maia Cherney To: Oganesyan, Vaheh References: <2ba9ce2f-c299-4ca9-a36a-99065d1b3...@unipd.it> <4d6faed8.7040...@ualberta.ca> <021001cbd9bc$f0ecc940$d2c65