Dear Dhiraj,
yes, on-column cleavage works as well quite efficiently with 3C protease.
I am performing it in a cold room (8-10C) for 1h normally reaches similar
efficiency.
Depending on your tags (if the 3C has a His-tag) you can add even more protease
1:50 then for sure 1h is more than enough.
T
Thanks Nikolay
I am doing on column cleavage, so I am incubating it
overnight at 4-degree C. Do you think even on column digestion is also going to
be done in one hour.
Thank you
Dhiraj
From: CCP4 bulletin board on behalf of Nikolay Dobr
