Sorry I got confused with the wrong labeling. But the stoichiometry issue and
poor expression is still the main problem. As Tom suggested, you should try to
improve the expression. Poor expression often results in impurities
copurifying. Also, if the interaction is not tight, why are you co-expr
Hi Dilip
It seems like your problem is poor stoichiometry. 37 kda protein
is poorly expressed. I wouldn’t be worried too much about 60 kda protein.
At-least at this stage. I might be mistaken but based on previous post, it
seems like your protein was aggregated. If it is so, you ne
