Dear Patrick,
Thank you for the insight!
Regards
Kavya
On 2024-02-06 00:29, Patrick Shaw Stewart wrote:
>> is it logical to say that if the protein is highly charged (either negative
>> or positive), it is likely to be more soluble and resist crystallization due
>> to electrostatic repu
Dear Guillaume,
That is right, if not for cations it would not have been possible.
We will check with the surface entropy reduction. Since we are designing
inhibitors for it, we cannot afford to do to much modifications.
Thank you
Regards
Kavya
On 2024-02-06 03:49, Guillaume Gaullie
Hello,
Regarding this question:
A curious question - is it logical to say that if the protein is highly charged
(either negative or positive), it is likely to be more soluble and resist
crystallization due to electrostatic repulsion? Our protein has highly
positively charged surface, although
Dear Kavya,
I wanted to share with you that we have faced the same issue with a few
projects. In addition to the great suggestions given earlier, I recommend
trying out fusion proteins, such as the surface entropy reduction MBP mutant,
or using protein binding partners like antibodies or natura
>
> is it logical to say that if the protein is highly charged (either
> negative or positive), it is likely to be more soluble and resist
> crystallization due to electrostatic repulsion?
Hi Kavyashreem
The project that I mentioned, where we looked at the crystallization
conditions reported in
Dear all,
Thank you all for your valuable experiences, inputs and references!! I
shall try them and hope for some good news! Its good to know there are
so many examples of crystallization at such high concentrations.
A curious question - is it logical to say that if the protein is highly
char
