[ccp4bb] co-crystallization

Hello,everyone, I have a question for cocrystallization, is there some relationship between Km value and substrate concentration when making cocrystallization? How can I know the substrate is enough for binding? Thank you very much! liuqing ___

[ccp4bb] inclusion bodies

Hello,everyone! Sorry to bother you! Now I have an enzyme cloned in pQE2 ,expressed in E.Coli M15 at room temperature,but there are a lot of inclusion bodies,perhaps 90%,it is a big trouble because I need to get much enzyme. Is there anyone knows how to solve? Thank you very