Re: [ccp4bb] Coot on MacOS Ventura

B&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > -- Wangsa Tirta Ismaya Jakarta, Indonesia ##

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

nopolized. Are any of you aware of > either empty columns or prepacked columns (e.g. metal affinity or ion > exchange resins) from other companies? > Thanks for your advice > > Markus > -- ======== Wangsa Tirta Ismaya Jakarta, Indonesia http://wangsatirtaismaya.blogspot.co.id

[ccp4bb]

method, the protein started > precipitating in most of the buffer conditions of Crystal screen and Peg > ion. The precipitation took place very quickly (within 5-10 mins). > How should I overcome this problem? > > > Regards > Prerana > -- Wangsa Tirta Ismaya Jakarta, Indonesia

Re: [ccp4bb] Inclusion bodies centrifugation

ls by sonication. > > > Can I do this by normal centrifugation? and if yes, at what speed? > > > Our centrifuge has maximum speed of 14000 rpm. Can we do the separation > using this centrifuge and if so how. > > > > Thanking in anticipation. > > > > > -- Wangsa Tirta Ismaya Utrecht, The Netherlands

Re: [ccp4bb] LSQKAB, version 6.0 vs version 6.1

he > > >>> treatment of residues with alternative conformations. > > >>> > > >>> This problem is not specific to a particular coordinates > > file. For > > >>> example, I can reproduce it using PDB entry 1QG3 and superposing > > >>> residues 1127-1318 of molecule A onto the same range of > > molecule B. > > >>> > > >>> I would appreciate if someone could enlighten me whether > > this is a > > >>> new FEATURE of ver 6.1 or a BUG; and how can this be > > avoided (i.e. > > >>> include residues with alternative conformations for calculations). > > >>> > > >>> Finally, I am running CCP4 6.1.0 in a Linux box with Suse > > 10.2 (Linux > > >>> 2.6.18.8-0.10-default i686). > > >>> > > >>> Happy holidays and happy New Year > > >>> Cheers > > >>> > > >>> Jose > > >>> > > >>> -- > > >>> > > >>> Jose M de Pereda, PhD > > >>> Instituto de Biologia Molecular y Celular del Cancer (IBMCC) > > >>> Spanish National Research Council - University of Salamanca > > >>> Campus Unamuno s/n > > >>> E-37007 Salamanca, Spain > > >>> Phone: +34-923-294819 > > >>> Fax:+34-923-294795 > > >>> http://xtal.cicancer.org/ > > >>> > > >>> > > >> > > > > > > -- Wangsa Tirta Ismaya = Josef-Israelstraat 66, 9718 GN Groningen The Netherlands

Re: [ccp4bb] AW: [ccp4bb] suggestions for UV spectrometer

>> Drexel University College of Medicine >> Room 10-102 New College Building >> 245 N. 15th St., Mailstop 497 >> Philadelphia, PA 19102-1192 USA >> >> (215) 762-7706 >> [EMAIL PROTECTED] >> >> >> >> >> -- >> Filip Van Petegem, PhD >> Assistant Professor >> The University of British Columbia >> Dept. of Biochemistry and Molecular Biology >> 2350 Health Sciences Mall - Rm 2.356 >> Vancouver, V6T 1Z3 >> >> phone: +1 604 827 4267 >> email: [EMAIL PROTECTED] >> http://crg.ubc.ca/VanPetegem/ >> > > . > B. Martin Hallberg, PhD > Assistant professor > Department of Cell and Molecular Biology > Medical Nobel Institute > Karolinska Institutet > Von Eulersv. 1 > SE-171 77 Stockholm > Sweden > Fax: +46-8-339380 > -- Wangsa Tirta Ismaya = Josef-Israelstraat 66, 9718 GN Groningen The Netherlands

[ccp4bb] Phaser job on CCP4 suite

t close the run option setting window (and click rerun to open a new run option setting window). Has anyone experience the same problem or could this be a bug? we are using the CCP4 program suite 6.1.0 (CCP4interface 2.0.3). Many thanks in advance, Wangsa -- Wangsa Tirta Ismaya ==