Or maybe a problem of oxidation?
I had a Se-met crystal that could be crystallized and diffracted just like the
native one, but only in the presence of the reducing agent (TCEP), no crystal
otherwise.
Tc
寄件人﹕ Frederic VELLIEUX
收件人﹕ CCP4BB@JISCMAIL.AC.UK
傳送日
Hi all,
Pseudo translational symmetry problem was detected in my crystal. I am going to
summarize my work on this in presentation and thesis. While some of my
examiners are not crystallographer, and I notice there are several good papers
on this, but I wonder is there any way to present the pr
roze these crystals- one should also try putting a crystal
in the beam without cryo-cooling, as cryo-cooling can often be detrimental to
diffraction.
Cheers, tom
From:CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of tat cheung
cheng
Sent: Monday, 19 Ap
then run them on a SDS-gel, then you will know for
sure if it's protein or not.
J僡gen
On Apr 18, 2010, at 10:46 PM, tat cheung cheng wrote:
Hi all,
>
>I have got some crystals, the purified protein was in Tris buffer with 300mM
>NaCl for crystallization. they grew in light we
Hi all,
I have got some crystals, the purified protein was in Tris buffer with 300mM
NaCl for crystallization. they grew in light weight PEG, PEG400 or monomethyl
ethyl PEG500, they were needle shaped, could be long (~0.2mm) but very thin all
the time and sometimes grew into sea-urchin like ne
Hi all,
I am now preparing to label my protein with selenium-methionine for phasing. As
I have heard that selenium-methionine is prone to oxidation, so is it a must to
add reducing agent all along the purification? if so, which reducing agent and
what concentration is better ? and any alternati
Hi all
I am now trying to do bromide soaking, but i am not really sure does the
bromide atom enter my crystal. So is there any signs that indicate the entry of
bromide atom? e.g. does the space group, cell dimension change? or just nothing
change, and the bromide atom just get in?
Thanks very
